Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Article Title: Host Immune Markers Distinguish Clostridioides difficile Infection From Asymptomatic Carriage and Non– C. difficile Diarrhea
doi: 10.1093/cid/ciz330
Figure Lengend Snippet: Dot plots showing the distribution of GCSF concentrations (pg/mL) in 6 cohorts: patients with symptomatic CDI (CDI-NAAT, defined by positive stool NAAT result and new-onset diarrhea), asymptomatic carriers (Carrier-NAAT, defined by positive stool NAAT result and absence of diarrhea), NAAT-negative patients with diarrhea (Diarrhea, NAAT-neg) and without diarrhea (No diarrhea, NAAT-neg), and symptomatic (CDI-Tox20) versus asymptomatic (Carrier-Tox20) cohorts (subsets of CDI-NAAT and Carrier-NAAT, defined by positive stool NAAT result and stool toxin A+B ≥20 pg/mL by Simoa). The bottom and top edges of the boxes for each cohort indicate the IQR, the horizontal line bisecting the box indicates the median value, and the whiskers represent values up to 1.5 IQR. The inset table indicates P values for comparison of median values for paired cohorts as indicated. Abbreviations: CDI, Clostridioides difficile infection; GCSF, granulocyte colony-stimulating factor; IQR, interquartile range; n/a, not applicable; NAAT, nucleic acid amplification test; neg, negative; Simoa, Single Molecule Array; Tox20, CDI-NAAT or Carrier-NAAT cohorts who had stool toxin A+B concentrations of ≥20 pg/mL.
Article Snippet: Clostridioides difficile toxin B (Native Antigen Company) coated on a Solid Phase Receptacle was used to capture anti–toxin B Ig present in the sample.
Techniques: Comparison, Infection, Amplification